| Gene | N28178 |
| Chromosome | 4 |
| Deletion Start | 42,933,603 |
| Deletion End | 42,939,606 |
| Deletion Size | 6,004 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 42,933,880 | 42,933,899 | CGTCGAGCCTGAGGAGTTTG |
| TUP | 42,933,901 | 42,933,921 | CAGGACCAGCCGATTCACGCC |
| TUR | 42,933,946 | 42,933,966 | GGTTTGTCATCGTCCAGCTTC |
| TDF | 42,938,896 | 42,938,915 | TGCCCGTGAACTAAGCAGAG |
| TDP | 42,938,918 | 42,938,943 | CAGCAATCCTATCGGCAGTCTGTGGG |
| TDR | 42,938,949 | 42,938,971 | GCGGCAGAGAATTTAGGACAGAG |
PCR Primers
| Primer | Sequence |
| SU | CTATGTTATCCTCATGACAACC |
| SD | TGCACACACGTATGCGTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 338 bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 363 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10311A-H2 | VGB6 | C57BL/6NTac | |
| 10311C-G7 | VGB6 | C57BL/6NTac | |
| 10311D-D7 | VGB6 | C57BL/6NTac | |
| 10311E-F10 | VGB6 | C57BL/6NTac | |
| 10311E-F4 | VGB6 | C57BL/6NTac | |
