| Gene | Nmb |
| Chromosome | 7 |
| Deletion Start | 80,904,974 |
| Deletion End | 80,902,441 |
| Deletion Size | 2,534 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,904,476 | 80,904,493 | TATCCTCCAGCGGGTCAG |
| TUP | 80,904,446 | 80,904,471 | AGCTCAGGATGTCAGCTTTGCCTCCA |
| TUR | 80,904,391 | 80,904,412 | GCTCTCACAGAGGGAAGACAAG |
| TDF | 80,903,420 | 80,903,440 | GGCTTGACTCACTGCTGGTTG |
| TDP | 80,903,392 | 80,903,418 | AAAGCATTGACCTGTGGTGCTCAAGGC |
| TDR | 80,903,354 | 80,903,372 | AACGGAGGAGCTGCCACAC |
PCR Primers
| Primer | Sequence |
| SU | GACGATGCCATAAGCACGC |
| SD | GACATTCTGTGTAAAGAGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 329 bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | 319 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10312A-C7 | VGB6 | C57BL/6NTac | |
| 10312B-A8 | VGB6 | C57BL/6NTac | |
| 10312B-B2 | VGB6 | C57BL/6NTac | |
