| Gene | Krt9 |
| Chromosome | 11 |
| Deletion Start | 100,193,200 |
| Deletion End | 100,187,059 |
| Deletion Size | 6,142 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,192,274 | 100,192,297 | CTTATCCTTCCTGTCCCATTTCTC |
| TUP | 100,192,245 | 100,192,273 | TTGGGCTGGTCACTTAATCTTCTCAGGTA |
| TUR | 100,192,221 | 100,192,240 | CACCCTGCACATCTGAGTTC |
| TDF | 100,187,702 | 100,187,720 | GTTCTCGGTGCCCATTGTG |
| TDP | 100,187,670 | 100,187,699 | TTGCAACTATCCATAACTCCAGTTCCAGGG |
| TDR | 100,187,632 | 100,187,651 | GCCTGGTGCTCTCAGACATC |
PCR Primers
| Primer | Sequence |
| SU | TGGGGCTTCTCTGCTGCTC |
| SD | TCAGGACAAGGAAGGGAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 305 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 333 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10313A-A9 | VGB6 | C57BL/6NTac | |
| 10313A-A11 | VGB6 | C57BL/6NTac | |
| 10313A-B1 | VGB6 | C57BL/6NTac | |
| 10313A-C1 | VGB6 | C57BL/6NTac | |
| 10313A-C10 | VGB6 | C57BL/6NTac | |
| 10313A-E7 | VGB6 | C57BL/6NTac | |
