| Gene | Mid1ip1 |
| Chromosome | X |
| Deletion Start | 10,718,216 |
| Deletion End | 10,718,824 |
| Deletion Size | 609 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,718,266 | 10,718,287 | TCGCTCTTTAACGCCATGAATC |
| TUP | 10,718,290 | 10,718,314 | TTCATTGGCGCGGTGAACAACATGG |
| TUR | 10,718,323 | 10,718,342 | AGCAGACTGGGCACCATCAC |
| TDF | 10,718,656 | 10,718,676 | GAGCAGCAGTTCCACTACCAC |
| TDP | 10,718,682 | 10,718,701 | CGGGCTGCACACCGTGCTCT |
| TDR | 10,718,738 | 10,718,757 | GCCGATCTCCTGCTTGTATC |
PCR Primers
| Primer | Sequence |
| SU | GATGCCGGGCATCCTAACC |
| SD | CAGGCTGAATGAAGGACATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 421 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | 307 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10328A-C7 | VGB6 | C57BL/6NTac | |
| 10328A-G6 | VGB6 | C57BL/6NTac | |
| 10328A-H7 | VGB6 | C57BL/6NTac | |
| 10328B-D6 | VGB6 | C57BL/6NTac | |
