| Gene | Chst14 |
| Chromosome | 2 |
| Deletion Start | 118,926,637 |
| Deletion End | 118,927,783 |
| Deletion Size | 1,147 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 118,927,092 | 118,927,113 | CCGCTTCCTCTACTGCTATGTC |
| TUP | 118,927,114 | 118,927,138 | CCCAAAGTGGCCTGCTCTAACTGGA |
| TUR | 118,927,149 | 118,927,166 | ATGCCAGCCAGCACCTTC |
| TDF | 118,927,494 | 118,927,513 | TGTGTACCACCTGTGCCAAC |
| TDP | 118,927,516 | 118,927,540 | TGTGCTGTGCACTACGACTTCGTGG |
| TDR | 118,927,545 | 118,927,565 | TCAGCCTCCAGCCTCTCATAG |
PCR Primers
| Primer | Sequence |
| SU | AGGCTCATGAGTTCTCTCCC |
| SD | GCAGACACGGATACCACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 405 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 320 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10331C-G1 | VGB6 | C57BL/6NTac | |
| 10331C-E4 | VGB6 | C57BL/6NTac | |
