| Gene | Efhd2 |
| Chromosome | 4 |
| Deletion Start | 141,874,864 |
| Deletion End | 141,859,726 |
| Deletion Size | 15,139 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 141,874,228 | 141,874,247 | TACCGACCTCCTTCCCACTG |
| TUP | 141,874,200 | 141,874,225 | AATGTTACTGCACCCACCACCCTACA |
| TUR | 141,874,166 | 141,874,185 | ACCGGCCCTTTCTCCCTTTC |
| TDF | 141,860,563 | 141,860,582 | GCGTTAAGGGTGCCAAGAAC |
| TDP | 141,860,538 | 141,860,561 | TCTTCGAGGCCAAGGTGAGAAGCC |
| TDR | 141,860,492 | 141,860,513 | GTCCTGTCTTAGCTGTGTCGTC |
PCR Primers
| Primer | Sequence |
| SU | GGAACGATGATGAGGAGCTG |
| SD | CATCACAGCCGCATGAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 354 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | 295 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10332A-C6 | VGB6 | C57BL/6NTac | |
| 10332A-D1 | VGB6 | C57BL/6NTac | |
| 10332B-D10 | VGB6 | C57BL/6NTac | |
