| Gene | Slc7a13 |
| Chromosome | 4 |
| Deletion Start | 19,818,785 |
| Deletion End | 19,841,591 |
| Deletion Size | 22,807 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 19,819,308 | 19,819,326 | GGTGGGCATACTCGGTGTC |
| TUP | 19,819,327 | 19,819,351 | ATTTCCCTCAGCGGCCTGTTCTTGC |
| TUR | 19,819,387 | 19,819,407 | CTCGGCATCAAACGCATTCTG |
| TDF | 19,841,365 | 19,841,383 | CGTTGGGCATCACCCTGAG |
| TDP | 19,841,390 | 19,841,416 | TTTGATCCCGCTTGTCAAGTCTCCAAA |
| TDR | 19,841,439 | 19,841,459 | CAGCCCACTGAGAAGGAAGAG |
PCR Primers
| Primer | Sequence |
| SU | CATTTCCAAACTCTGGTTCC |
| SD | AATCCAGTAGAGGATATTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 377 bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | 363 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10337A-H4 | VGB6 | C57BL/6NTac | |
| 10337A-H6 | VGB6 | C57BL/6NTac | |
| 10337A-D1 | VGB6 | C57BL/6NTac | |
