| Gene | Hils1 |
| Chromosome | 11 |
| Deletion Start | 94,967,864 |
| Deletion End | 94,968,393 |
| Deletion Size | 530 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,967,892 | 94,967,911 | GGTGGCTGGAGACCAAGATG |
| TUP | 94,967,913 | 94,967,932 | CGGCACACTGTGGGTCCCAA |
| TUR | 94,967,961 | 94,967,980 | GCAGGCTTTGGGTACTGATG |
| TDF | 94,968,187 | 94,968,204 | TCCCTCTGCCTGTGCAAG |
| TDP | 94,968,208 | 94,968,230 | CGGGCCCTCAAGTCCAACCACAG |
| TDR | 94,968,253 | 94,968,272 | TGAGGCTTCTGGCTCTTCTG |
PCR Primers
| Primer | Sequence |
| SU | CTAGAGAGGCGCCTCTGAGG |
| SD | TGTGGCTGGCCAAGCTGCTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 292 bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | 326 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10339A-C11 | VGB6 | C57BL/6NTac | |
| 10339A-D11 | VGB6 | C57BL/6NTac | |
| 10339A-D12 | VGB6 | C57BL/6NTac | |
| 10339A-F2 | VGB6 | C57BL/6NTac | |
| 10339A-H6 | VGB6 | C57BL/6NTac | |
| 10339A-H4 | VGB6 | C57BL/6NTac | |
