10341   Kcnk15     Status: ES cell colonies screened / QC positives

GeneKcnk15
Chromosome2
Deletion Start163,853,731
Deletion End163,858,874
Deletion Size5,144
Genome Build38
MGI IDMGI:2675209
NCBI Gene ID241769
BAC ID274F18
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF163,853,835163,853,854CGCTCATTCTGTGCATCCTG
TUP163,853,855163,853,875TCCTACCTGCTGGTGGGTGCC
TUR163,853,955163,853,973GCGGAGAAGCGGTACTTTC
TDF163,858,208163,858,226GGACTCGGGCAAAGTGTTC
TDP163,858,227163,858,250TGCATGTTCTATGCCCTCCTGGGC
TDR163,858,266163,858,284CCAGGCTCTGGAAGGTGAC

PCR Primers

PrimerSequence
SUCATTTGTCATCCATCCCTGC
SDGATGCTTGACCTGGGTATCG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev399 bp-++
TUFTUR139 bp++-
TDFTDR77 bp++-
NeoFwdSD338 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
10341A-F11VGB6C57BL/6NTac
10341B-D7VGB6C57BL/6NTac
10341B-G11VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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