| Gene | Ly6d |
| Chromosome | 15 |
| Deletion Start | 74,763,557 |
| Deletion End | 74,762,337 |
| Deletion Size | 1,221 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 74,763,193 | 74,763,211 | TTACTGCCTTTGCCACCTC |
| TUP | 74,763,164 | 74,763,188 | CTGTCCTGGGCAACACTAGGGTGGT |
| TUR | 74,763,136 | 74,763,156 | CTGAAGAGCCCGGAGAAAGAG |
| TDF | 74,762,768 | 74,762,789 | GCCCGTCCAACTTCTACTTCTG |
| TDP | 74,762,737 | 74,762,758 | CACCTCAGGTGAGTGGCAGCCC |
| TDR | 74,762,685 | 74,762,704 | GGCCCATTCCCAGGATCAAG |
PCR Primers
| Primer | Sequence |
| SU | AATGGTCTGCCAGGCTTCTG |
| SD | AAACTTACTATCCCACTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 366 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | 420 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10343C-A12 | VGB6 | C57BL/6NTac | |
| 10343C-B1 | VGB6 | C57BL/6NTac | |
