| Gene | Ndufaf4 |
| Chromosome | 4 |
| Deletion Start | 24,898,150 |
| Deletion End | 24,901,998 |
| Deletion Size | 3,849 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,898,268 | 24,898,286 | TTCTACCCGCGACCTCCTG |
| TUP | 24,898,294 | 24,898,316 | ACCGGAGTCGTGAGTGCAGTCCC |
| TUR | 24,898,329 | 24,898,348 | GGGACGACCTCAATGTCACC |
| TDF | 24,901,040 | 24,901,061 | GACCAGAAGCGTCTTACACATC |
| TDP | 24,901,072 | 24,901,099 | TTTATCAGTGAGGGAAGGCTGGAAGACA |
| TDR | 24,901,126 | 24,901,147 | TCGAATGAGCAGCACTCTAACG |
PCR Primers
| Primer | Sequence |
| SU | GGGATCACAGCAGCTCATC |
| SD | GGCCACATGATCAATACAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 383 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | 264 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10351A-E12 | VGB6 | C57BL/6NTac | |
| 10351A-G8 | VGB6 | C57BL/6NTac | |
