| Gene | Evi2b |
| Chromosome | 11 |
| Deletion Start | 79,516,744 |
| Deletion End | 79,515,413 |
| Deletion Size | 1,351 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,516,521 | 79,516,541 | AGCATCACCTGCCCAAATCAC |
| TUP | 79,516,494 | 79,516,520 | TCCTGAACAAGCAACACCAGCTGTTTA |
| TUR | 79,516,419 | 79,516,441 | GTTTGAGGCAAGGAGTTGTTGAC |
| TDF | 79,515,477 | 79,515,496 | GCTCAATGCCAGGAGTTCTC |
| TDP | 79,515,451 | 79,515,475 | ACTCCTGACCTCCATCCAGACCTCA |
| TDR | 79,515,416 | 79,515,435 | CAGCAGCTCTGTAGGTGGAG |
PCR Primers
| Primer | Sequence |
| SU | GAAGCTGAGGAACCTAGAGG |
| SD | GTAGCTGGTAAGCATACCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 382 bp | - | + | + |
| TUF | TUR | 123 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | 283 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10352A-E1 | VGB6 | C57BL/6NTac | |
