| Gene | Prl7b1 |
| Chromosome | 13 |
| Deletion Start | 27,610,519 |
| Deletion End | 27,601,880 |
| Deletion Size | 8,640 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 27,610,110 | 27,610,127 | TGGGACCTTGAGCCTGAC |
| TUP | 27,610,082 | 27,610,109 | TGGCCAGCCAGTCCAATTCATTTAGAAA |
| TUR | 27,610,059 | 27,610,081 | AGAAACTCTCACTACCCTGAAAC |
| TDF | 27,602,876 | 27,602,897 | TGTGTAGCTGCCAGACTTTATG |
| TDP | 27,602,836 | 27,602,861 | TATACTACGAGCCTGGGACAACCCTC |
| TDR | 27,602,807 | 27,602,830 | ACTCTTGAGTTCTGTCTCCATGTG |
PCR Primers
| Primer | Sequence |
| SU | TGGATCATGAAGTCAACTTGG |
| SD | AAAGTTTCCAGCAACTGTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 368 bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | 343 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10354A-B5 | VGB6 | C57BL/6NTac | |
| 10354A-F5 | VGB6 | C57BL/6NTac | |
| 10354A-H4 | VGB6 | C57BL/6NTac | |
| 10354B-E5 | VGB6 | C57BL/6NTac | |
| 10354B-H9 | VGB6 | C57BL/6NTac | |
