| Gene | Hbq1a |
| Chromosome | 11 |
| Deletion Start | 32,300,101 |
| Deletion End | 32,301,520 |
| Deletion Size | 1,420 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,300,604 | 32,300,629 | GTTCTCTTTCTTCAGTTCTTCAGCTG |
| TUP | 32,300,630 | 32,300,652 | CTGTCTGCTGGTGACTCTCGCCA |
| TUR | 32,300,705 | 32,300,727 | AAAAGTCACATGACCCAGACACG |
| TDF | 32,301,271 | 32,301,292 | CATGTGTGCTTGGCATGTAGTG |
| TDP | 32,301,299 | 32,301,323 | TACATGTGTGTCTGGTGTGTGTGGC |
| TDR | 32,301,366 | 32,301,389 | ACAGTGGCATAAAACCCCATAAAC |
PCR Primers
| Primer | Sequence |
| SU | TGACAGTGCCATATGGACAC |
| SD | ACAGCAGCATCATACTCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 368 bp | - | + | + |
| TUF | TUR | 124 bp | + | + | - |
| TDF | TDR | 119 bp | + | + | - |
| NeoFwd | SD | 326 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10358A-A1 | VGB6 | C57BL/6NTac | |
| 10358A-A9 | VGB6 | C57BL/6NTac | |
| 10358A-A11 | VGB6 | C57BL/6NTac | |
| 10358A-C6 | VGB6 | C57BL/6NTac | |
| 10358A-G11 | VGB6 | C57BL/6NTac | |
