| Gene | Ccdc42 |
| Chromosome | 11 |
| Deletion Start | 68,587,211 |
| Deletion End | 68,597,907 |
| Deletion Size | 10,697 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 68,587,570 | 68,587,588 | TGCTCCCTACCCGATCCTG |
| TUP | 68,587,590 | 68,587,612 | TCCTGCCTCTCAGAGGGTCCTGT |
| TUR | 68,587,622 | 68,587,642 | AACTTCCTGCCCGAGAACAAC |
| TDF | 68,597,096 | 68,597,113 | TCCCAGCCCTGACCTTAC |
| TDP | 68,597,114 | 68,597,136 | TGCGCTAACTGAGTCCACGGTCA |
| TDR | 68,597,176 | 68,597,195 | TCCCAAACCAGTGCCACAAG |
PCR Primers
| Primer | Sequence |
| SU | ACTAAAGCAGCCTCTAGCTG |
| SD | TGGCATCTGGCTTTCCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 313 bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | 269 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10362A-D12 | VGB6 | C57BL/6NTac | |
| 10362A-F2 | VGB6 | C57BL/6NTac | |
| 10362B-D2 | VGB6 | C57BL/6NTac | |
