| Gene | Hpdl |
| Chromosome | 4 |
| Deletion Start | 116,821,259 |
| Deletion End | 116,820,126 |
| Deletion Size | 1,134 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,820,905 | 116,820,923 | GGCACAGCCACCTACACAG |
| TUP | 116,820,883 | 116,820,904 | TGCTCAGCTCTCCGGCTGGTAA |
| TUR | 116,820,841 | 116,820,859 | GATCCGCGATAGCCAGCAC |
| TDF | 116,820,410 | 116,820,428 | CGGCTTCTGACTCCTCCTG |
| TDP | 116,820,386 | 116,820,409 | AAGCTTATTACCAGCAGCCCGGCA |
| TDR | 116,820,360 | 116,820,380 | CCCTGCGGCTAGAATTTGTTC |
PCR Primers
| Primer | Sequence |
| SU | ATACAATTTACCCTGCAGAGCC |
| SD | AAATGCAGGAAGACACAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 362 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | 352 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10364A-A3 | VGB6 | C57BL/6NTac | |
| 10364A-C3 | VGB6 | C57BL/6NTac | |
| 10364A-E9 | VGB6 | C57BL/6NTac | |
| 10364A-H6 | VGB6 | C57BL/6NTac | |
