| Gene | Lmf2 |
| Chromosome | 15 |
| Deletion Start | 89,355,446 |
| Deletion End | 89,351,570 |
| Deletion Size | 3,877 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,354,579 | 89,354,597 | GCTGCTCTGGGTTGCATAC |
| TUP | 89,354,551 | 89,354,575 | TCAGCCTACCAGGCAAGTGAGACAC |
| TUR | 89,354,522 | 89,354,544 | GGTGGGTTAGATGAAGGCTGAAG |
| TDF | 89,352,546 | 89,352,564 | TCCAGCCATCCCAGCTAAC |
| TDP | 89,352,520 | 89,352,545 | CATGTGTCCCTCTGTCAGGAGATCGA |
| TDR | 89,352,491 | 89,352,510 | GGCTCACATTCCCAGGTTTG |
PCR Primers
| Primer | Sequence |
| SU | ACTAAGTTTCTAGGTGGTTAG |
| SD | GGCCGCAGGCCCACTTCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 274 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 334 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10366A-F7 | VGB6 | C57BL/6NTac | |
| 10366A-F9 | VGB6 | C57BL/6NTac | |
| 10366B-D3 | VGB6 | C57BL/6NTac | |
| 10366B-H1 | VGB6 | C57BL/6NTac | |
| 10366B-G10 | VGB6 | C57BL/6NTac | |
