10368   Gh     Status: ES cell colonies screened / QC positives

GeneGh
Chromosome11
Deletion Start106,301,746
Deletion End106,300,347
Deletion Size1,400
Genome Build38
MGI IDMGI:95707
NCBI Gene ID14599
BAC ID290K6
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF106,301,209106,301,229TGACCACCCTTCTTCCAGGAG
TUP106,301,186106,301,208CGTGCCTACATTCCCGAGGGACA
TUR106,301,153106,301,172AAAGCAGCCTGGGCATTCTG
TDF106,300,809106,300,828CACCTCGGACCGTGTCTATG
TDP106,300,779106,300,806AAACTGAAGGACCTGGAAGAGGGCATCC
TDR106,300,754106,300,773TCCATCCTCACCTGCATCAG

PCR Primers

PrimerSequence
SUCGATGAGACGATAAGGTCAG
SDCATCAGCTCACAGGATTCTC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev374 bp-++
TUFTUR77 bp++-
TDFTDR75 bp++-
NeoFwdSD268 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
10368A-B4VGB6C57BL/6NTac
10368A-C8VGB6C57BL/6NTac
10368A-D3VGB6C57BL/6NTac
10368A-E4VGB6C57BL/6NTac
10368A-F1VGB6C57BL/6NTac
10368A-F10VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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