| Gene | Reg2 |
| Chromosome | 6 |
| Deletion Start | 78,405,498 |
| Deletion End | 78,407,999 |
| Deletion Size | 2,502 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,405,541 | 78,405,565 | TGTTCTTATGCCTGATGTTCCTGTC |
| TUP | 78,405,572 | 78,405,599 | CCAAGGTAAGCTTGTTGAACCATGACCC |
| TUR | 78,405,603 | 78,405,623 | GGAGCAAAGCCTTCATGTCTG |
| TDF | 78,407,628 | 78,407,645 | GAACCGTCGTTGGCACTG |
| TDP | 78,407,647 | 78,407,676 | AGCAGTGGCTCCCTATTTCTCTTCAAGTCA |
| TDR | 78,407,710 | 78,407,733 | TGAAGTCAGCGATACACAATAACC |
PCR Primers
| Primer | Sequence |
| SU | GAAGTACTGGTCTACCAGTG |
| SD | GAGAGATAAAGAATGCATGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 371 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | 328 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10369A-C1 | VGB6 | C57BL/6NTac | |
| 10369E-H2 | VGB6 | C57BL/6NTac | |
| 10369F-G3 | VGB6 | C57BL/6NTac | |
