| Gene | Odf4 |
| Chromosome | 11 |
| Deletion Start | 68,926,964 |
| Deletion End | 68,921,940 |
| Deletion Size | 5,025 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 68,926,800 | 68,926,818 | AGCAGGAACAGGCGGTCAC |
| TUP | 68,926,777 | 68,926,799 | TGGAGCATCGTCGCAACTCCCTA |
| TUR | 68,926,732 | 68,926,750 | GGCCATCCAGCGAGAGTTG |
| TDF | 68,922,111 | 68,922,130 | TGAGCAGCACCACCATCAAC |
| TDP | 68,922,086 | 68,922,110 | ACTGCTTGCAGCAGCTTAGGGCCAG |
| TDR | 68,922,066 | 68,922,084 | GGAGGGACTCACCAGAGAC |
PCR Primers
| Primer | Sequence |
| SU | CTGTCAGAGAGCATTTGGG |
| SD | AACAAACACATCGGGTTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 401 bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | 280 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10377B-A6 | VGB6 | C57BL/6NTac | |
| 10377B-A8 | VGB6 | C57BL/6NTac | |
| 10377B-B11 | VGB6 | C57BL/6NTac | |
| 10377B-D1 | VGB6 | C57BL/6NTac | |
| 10377B-G11 | VGB6 | C57BL/6NTac | |
| 10377B-B2 | VGB6 | C57BL/6NTac | |
