| Gene | Krt27 |
| Chromosome | 11 |
| Deletion Start | 99,351,070 |
| Deletion End | 99,345,560 |
| Deletion Size | 5,530 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,350,876 | 99,350,895 | GGGCCTTCACAGCGAATGAG |
| TUP | 99,350,853 | 99,350,874 | ACGGCCTCCTCTCCGGCAATGA |
| TUR | 99,350,788 | 99,350,806 | CCAGTGCTTGCACGTTCTC |
| TDF | 99,345,782 | 99,345,803 | CAGAGTTCACACCCTGGAAGAG |
| TDP | 99,345,753 | 99,345,778 | TCCACCAAAGTCAACAAGACCGAGCA |
| TDR | 99,345,718 | 99,345,738 | TCTGCTTTAGAGGCGGAGTTC |
PCR Primers
| Primer | Sequence |
| SU | CTTGATAACACTCCACCCTG |
| SD | CCCTGTGTATTCAACCAGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 375 bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | 282 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10380A-C1 | VGB6 | C57BL/6NTac | |
| 10380A-D5 | VGB6 | C57BL/6NTac | |
| 10380A-G2 | VGB6 | C57BL/6NTac | |
