| Gene | Dusp6 |
| Chromosome | 10 |
| Deletion Start | 99,263,695 |
| Deletion End | 99,266,363 |
| Deletion Size | 2,669 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,264,246 | 99,264,270 | TTGGGTAGGTGTAGGATTAAGGTTC |
| TUP | 99,264,271 | 99,264,299 | ACAAGAGCTGGAAACGTAGTTCAGGGTGC |
| TUR | 99,264,307 | 99,264,326 | TTTCGAGGAGCTGCGAGGTG |
| TDF | 99,266,026 | 99,266,046 | GCATTGCTTGGCAGGTATCAG |
| TDP | 99,266,055 | 99,266,075 | TCACCGTGACAGTGGCGTACC |
| TDR | 99,266,089 | 99,266,109 | GCATCGTTCATGGACAGGTTG |
PCR Primers
| Primer | Sequence |
| SU | TGAATAATCCGGCGAGAAAC |
| SD | AGATGCAACAGGAAACAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10381A-H2 | VGB6 | C57BL/6NTac | |
| 10381A-F5 | VGB6 | C57BL/6NTac | |
| 10381A-A7 | VGB6 | C57BL/6NTac | |
| 10381A-B7 | VGB6 | C57BL/6NTac | |
