| Gene | Krt15 |
| Chromosome | 11 |
| Deletion Start | 100,135,855 |
| Deletion End | 100,131,997 |
| Deletion Size | 3,859 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,135,412 | 100,135,432 | GATCGGGACTACAGCCATTAC |
| TUP | 100,135,384 | 100,135,410 | TTAAGACCATGGAAGAGATCCGGGACA |
| TUR | 100,135,348 | 100,135,367 | GAGCCACCTCCATCCATCTG |
| TDF | 100,132,919 | 100,132,938 | CCACGAGCTGTGTTGTTGAG |
| TDP | 100,132,891 | 100,132,918 | TGGAAAGAGACCTGAAGAGTCTGCAGTC |
| TDR | 100,132,856 | 100,132,879 | GCAATCTACCCAAAGTACCCAAAC |
PCR Primers
| Primer | Sequence |
| SU | TGAGTTCTGTGGAGGTGTGC |
| SD | TGAAGGAACAGCCGCCATAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 337 bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 307 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10386A-C4 | VGB6 | C57BL/6NTac | |
| 10386A-C7 | VGB6 | C57BL/6NTac | |
| 10386A-D3 | VGB6 | C57BL/6NTac | |
| 10386A-G7 | VGB6 | C57BL/6NTac | |
| 10386A-G12 | VGB6 | C57BL/6NTac | |
| 10386A-F5 | VGB6 | C57BL/6NTac | |
