| Gene | Fam58b |
| Chromosome | 11 |
| Deletion Start | 78,751,659 |
| Deletion End | 78,750,886 |
| Deletion Size | 774 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,751,396 | 78,751,415 | GCAAAGTGGAGGAGCAACAC |
| TUP | 78,751,364 | 78,751,389 | ACTCGTGACATCATCAACGTGTCGCA |
| TUR | 78,751,338 | 78,751,358 | GCTCACTGCCCGGGTTAAAGT |
| TDF | 78,751,170 | 78,751,191 | CTGAATCGTTACAGCTGGCAAC |
| TDP | 78,751,133 | 78,751,160 | TCTCAGTTACAGCTTGGGCTCTGCTACG |
| TDR | 78,751,088 | 78,751,104 | TGCTGAGCCTGGAAGCG |
PCR Primers
| Primer | Sequence |
| SU | TCAGGAACAGTGAAAAAGCC |
| SD | GTCCATGCCTCAAATGCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 372 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | 351 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10387A-C7 | VGB6 | C57BL/6NTac | |
| 10387A-D3 | VGB6 | C57BL/6NTac | |
| 10387A-D9 | VGB6 | C57BL/6NTac | |
| 10387A-H5 | VGB6 | C57BL/6NTac | |
| 10387A-B12 | VGB6 | C57BL/6NTac | |
| 10387A-D4 | VGB6 | C57BL/6NTac | |
