| Gene | Bpifa1 |
| Chromosome | 2 |
| Deletion Start | 154,143,750 |
| Deletion End | 154,148,090 |
| Deletion Size | 4,341 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 154,144,111 | 154,144,132 | AAGGCAGGTATGAGGGAGGAAG |
| TUP | 154,144,136 | 154,144,161 | AGGTAAGGACCTGCAGTTGGCAGACA |
| TUR | 154,144,174 | 154,144,197 | TGAGAGCTGTAAAGAGGGAAAGAG |
| TDF | 154,147,926 | 154,147,945 | CTGAGCTGGCTGGAGGAGAC |
| TDP | 154,147,957 | 154,147,981 | CCAACATTCCTGGGAGTGGTGAGCA |
| TDR | 154,147,989 | 154,148,011 | GGCATATTCTGGTGGTGAAGGAG |
PCR Primers
| Primer | Sequence |
| SU | AAACTGCCTAGAGTCGCTAG |
| SD | GTCTTTCATTACAGTGATAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 19593 bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | 238 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10389A-D7 | VGB6 | C57BL/6NTac | |
| 10389A-D10 | VGB6 | C57BL/6NTac | |
| 10389A-A5 | VGB6 | C57BL/6NTac | |
