| Gene | Gpr156 |
| Chromosome | 16 |
| Deletion Start | 37,947,704 |
| Deletion End | 37,979,104 |
| Deletion Size | 31,401 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,948,298 | 37,948,320 | GCTACTTCTGGTGCTCTTCTTTC |
| TUP | 37,948,321 | 37,948,344 | TGGCTTTCACAATTCGCTGCAGGA |
| TUR | 37,948,346 | 37,948,364 | GGAGGCTGCTTACCTGTTC |
| TDF | 37,978,989 | 37,979,009 | CCTACTTTCACCCGCAGACAC |
| TDP | 37,979,011 | 37,979,035 | ACTGTCCTTGCTGTGCATTGGGACG |
| TDR | 37,979,042 | 37,979,061 | CCCAGGATAGGGCCAAACAC |
PCR Primers
| Primer | Sequence |
| SU | ACCAGCTGAAGAACATTTGC |
| SD | CCAGTCTCTCTGGGCATCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 351 bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 395 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10390C-B8 | VGB6 | C57BL/6NTac | |
| 10390D-H1 | VGB6 | C57BL/6NTac | |
| 10390D-F4 | VGB6 | C57BL/6NTac | |
