10391   Igsf6     Status: ES cell colonies screened / QC positives

GeneIgsf6
Chromosome7
Deletion Start121,074,502
Deletion End121,065,461
Deletion Size9,042
Genome Build38
MGI IDMGI:1891393
NCBI Gene ID80719
BAC ID261G1
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF121,074,288121,074,312GAGGTCACTTCTCCCACAGAATTAG
TUP121,074,248121,074,277ATAGCTGAAGAAAGGTTTCCTACCGGCCCT
TUR121,074,220121,074,242GATGTGCTCAAAGCAGCATCAAC
TDF121,065,941121,065,962TGGGAGGCTAAGGCAGAATTAC
TDP121,065,917121,065,940CAGCTTTAGCCAGCTTGGGCCCTA
TDR121,065,825121,065,843GGTTGCTCAGGACCACATC

PCR Primers

PrimerSequence
SUTCACGTCTAACAGTTTCTGG
SDGATGTGTCTCTGACTTCAGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR93 bp++-
TDFTDR138 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
10391A-B3VGB6C57BL/6NTac
10391A-F12VGB6C57BL/6NTac
10391A-G5VGB6C57BL/6NTac
10391A-G7VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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