| Gene | Gjd4 |
| Chromosome | 18 |
| Deletion Start | 9,282,615 |
| Deletion End | 9,279,963 |
| Deletion Size | 2,653 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 9,282,439 | 9,282,460 | CTGGCCTACCTCCAGAAGAAAG |
| TUP | 9,282,404 | 9,282,430 | ATCACTATTAGTGTGGGAGCCTTGGCA |
| TUR | 9,282,362 | 9,282,384 | TCTCCGATGTTGATGGGTACAAG |
| TDF | 9,280,844 | 9,280,862 | TGTCACCGCTGCGATTCTG |
| TDP | 9,280,820 | 9,280,841 | TAGTGCAGAGCCTGGCCTTGCT |
| TDR | 9,280,782 | 9,280,804 | GGTGTAGGGTGTAAGTGCCAAAG |
PCR Primers
| Primer | Sequence |
| SU | AGGGAGATGACTTCTGCTTC |
| SD | GCAGTTTAACAGAAAGTAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 341 bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | 309 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10392A-E7 | VGB6 | C57BL/6NTac | |
| 10392A-E9 | VGB6 | C57BL/6NTac | |
| 10392A-G7 | VGB6 | C57BL/6NTac | |
| 10392A-E12 | VGB6 | C57BL/6NTac | |
