| Gene | Dem1 |
| Chromosome | 4 |
| Deletion Start | 120,922,663 |
| Deletion End | 120,921,523 |
| Deletion Size | 1,141 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,922,557 | 120,922,580 | GGATCTGGAAGAAGCCAAAGAATC |
| TUP | 120,922,529 | 120,922,553 | TGTTTCACTTAGCAAGCCTGGCCCT |
| TUR | 120,922,502 | 120,922,522 | GTCATCCTTCCCAGGGAGTTC |
| TDF | 120,921,692 | 120,921,710 | GCAGCATTACGTGGCCTAC |
| TDP | 120,921,665 | 120,921,688 | ATGGGCCACCGAGATCCTCAAGGC |
| TDR | 120,921,606 | 120,921,628 | CACTCGCAGATGTCCACATAGTC |
PCR Primers
| Primer | Sequence |
| SU | GTGTTCTCCCTCTAGAAGTG |
| SD | AAGAATTTTCTTGAGCCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 440 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | 348 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10394A-E4 | VGB6 | C57BL/6NTac | |
| 10394A-G12 | VGB6 | C57BL/6NTac | |
| 10394A-H4 | VGB6 | C57BL/6NTac | |
| 10394B-C7 | VGB6 | C57BL/6NTac | |
| 10394B-F5 | VGB6 | C57BL/6NTac | |
| 10394A-C11 | VGB6 | C57BL/6NTac | |
