| Gene | A930005I04Rik |
| Chromosome | 5 |
| Deletion Start | 34,917,580 |
| Deletion End | 34,923,564 |
| Deletion Size | 5,985 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,917,675 | 34,917,694 | AAGTGCCTGGCTACCTTGTG |
| TUP | 34,917,697 | 34,917,719 | TCCTCAGACGGAGAAGCATCGGA |
| TUR | 34,917,735 | 34,917,752 | CGCGCATCTCTGCATCTG |
| TDF | 34,923,111 | 34,923,132 | CACTACCTGCTCCTCCCACAAG |
| TDP | 34,923,133 | 34,923,158 | CCAGCACTAGCTTTCACCAGCTGTTC |
| TDR | 34,923,193 | 34,923,212 | GGTCTCGGGTCTCTGCTCAC |
PCR Primers
| Primer | Sequence |
| SU | ACTGCCTAGATGCCCATGTAG |
| SD | AGTATCAGATAGAGACTATAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 469 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | 323 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10395A-B10 | VGB6 | C57BL/6NTac | |
| 10395A-D1 | VGB6 | C57BL/6NTac | |
| 10395A-D3 | VGB6 | C57BL/6NTac | |
| 10395A-F1 | VGB6 | C57BL/6NTac | |
| 10395A-D10 | VGB6 | C57BL/6NTac | |
| 10395A-E9 | VGB6 | C57BL/6NTac | |
