| Gene | Krt35 |
| Chromosome | 11 |
| Deletion Start | 100,096,093 |
| Deletion End | 100,092,508 |
| Deletion Size | 3,586 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,095,765 | 100,095,785 | GCAGCAGGTGCCCTATATGTG |
| TUP | 100,095,736 | 100,095,763 | CCCGATTACCAGTCTTACTTCCGGACCA |
| TUR | 100,095,693 | 100,095,713 | TGAAGCCACTCTGAACCACTC |
| TDF | 100,093,435 | 100,093,453 | AACACGCCCTGGTAGTAAC |
| TDP | 100,093,412 | 100,093,433 | CCTCTGTGCAGGTGCAGTCACC |
| TDR | 100,093,358 | 100,093,378 | GATCCAGACAGCATGTCCTTC |
PCR Primers
| Primer | Sequence |
| SU | GGCCAACCAACACCCACTG |
| SD | GCCCGTTGAAATGATGAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 338 bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | 297 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10400A-A4 | VGB6 | C57BL/6NTac | |
| 10400A-B4 | VGB6 | C57BL/6NTac | |
| 10400A-E12 | VGB6 | C57BL/6NTac | |
| 10400B-B8 | VGB6 | C57BL/6NTac | |
| 10400B-D6 | VGB6 | C57BL/6NTac | |
| 10400B-F7 | VGB6 | C57BL/6NTac | |
