| Gene | Ctdnep1 |
| Chromosome | 11 |
| Deletion Start | 69,981,469 |
| Deletion End | 69,990,085 |
| Deletion Size | 8,617 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,981,870 | 69,981,891 | AGAAAGAAGAGGCCGGGAACAC |
| TUP | 69,981,897 | 69,981,925 | TGCTGGTAGCCAGAACTATTGATATGGGC |
| TUR | 69,981,959 | 69,981,979 | CCACCCACACCACTGACAAAG |
| TDF | 69,989,885 | 69,989,906 | GTTCTGTTCTGTCCAGTGAGAC |
| TDP | 69,989,912 | 69,989,939 | TGTGCTAGTGTTGTGAGGGTGTCAACTA |
| TDR | 69,989,967 | 69,989,990 | CATCAGCAGTGAACCTGAAATGAC |
PCR Primers
| Primer | Sequence |
| SU | CCTAGCCCGACCTGTTTCG |
| SD | CACCAGACTGTTTCGGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 405 bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | 302 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10405A-D10 | VGB6 | C57BL/6NTac | |
| 10405A-F11 | VGB6 | C57BL/6NTac | |
| 10405A-G4 | VGB6 | C57BL/6NTac | |
| 10405A-G7 | VGB6 | C57BL/6NTac | |
| 10405A-G12 | VGB6 | C57BL/6NTac | |
| 10405A-B9 | VGB6 | C57BL/6NTac | |
