| Gene | Kif12 |
| Chromosome | 4 |
| Deletion Start | 63,172,002 |
| Deletion End | 63,165,784 |
| Deletion Size | 6,219 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 63,171,969 | 63,171,990 | TCTCCCGATGGGTAAGTACAAC |
| TUP | 63,171,941 | 63,171,967 | TGTTTGATGTTGCGGATACCCTGAAGC |
| TUR | 63,171,911 | 63,171,930 | CCCTGCCTTTAGTCGCTGTC |
| TDF | 63,166,601 | 63,166,620 | GCTCTGTCCTTGGCATCTTG |
| TDP | 63,166,576 | 63,166,600 | TGCAACAGGCATGGCTAGACTCAGC |
| TDR | 63,166,533 | 63,166,551 | CAGGTGGCCTCAGCCTTTC |
PCR Primers
| Primer | Sequence |
| SU | GATCCTTAGACTCTAGAAACC |
| SD | TCTTTCACCTCAATGATCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 350 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | 327 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10408A-B9 | VGB6 | C57BL/6NTac | |
| 10408A-C7 | VGB6 | C57BL/6NTac | |
