| Gene | Ovca2 |
| Chromosome | 11 |
| Deletion Start | 75,178,792 |
| Deletion End | 75,177,875 |
| Deletion Size | 918 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,178,550 | 75,178,571 | TCTCACCTAGCGCCTTTAACTC |
| TUP | 75,178,526 | 75,178,549 | CGGCTCTAGTCTGAGCTCCCAGGT |
| TUR | 75,178,492 | 75,178,511 | ACCTGGAATGGCCTGGACAC |
| TDF | 75,177,953 | 75,177,974 | CACTCCGGTGGTCACTTCATTC |
| TDP | 75,177,926 | 75,177,949 | CAGCTGCATCTCAGCGTCAGGCCT |
| TDR | 75,177,898 | 75,177,918 | TCTGCAAACTGGTCCAAGAAC |
PCR Primers
| Primer | Sequence |
| SU | TAGAGAGACTTGAATCTATC |
| SD | TTGGTTTGGTGCTGCCCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 383 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 324 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10413C-A5 | VGB6 | C57BL/6NTac | |
| 10413C-F9 | VGB6 | C57BL/6NTac | |
