| Gene | Klhl11 |
| Chromosome | 11 |
| Deletion Start | 100,472,725 |
| Deletion End | 100,462,837 |
| Deletion Size | 9,889 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,472,508 | 100,472,528 | AGAGGCCGAGGACTTCGAGTG |
| TUP | 100,472,479 | 100,472,502 | CTCACTGCTCCGAGTTGTCCTGGC |
| TUR | 100,472,431 | 100,472,452 | ACAGGGTGATGTCGCAGAACAG |
| TDF | 100,462,930 | 100,462,948 | GAAGGACCGCATCCGACAG |
| TDP | 100,462,904 | 100,462,929 | ATGCAAGAGATACATCGGCACGCCCT |
| TDR | 100,462,882 | 100,462,903 | ACTTGGCACTCTCCGCATGTTC |
PCR Primers
| Primer | Sequence |
| SU | CACTCCAAAGCAACATAAAGTAG |
| SD | AAGGAGTTAGTGGTTGGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 375 bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | 370 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10416A-A2 | VGB6 | C57BL/6NTac | |
| 10416A-A3 | VGB6 | C57BL/6NTac | |
| 10416A-A6 | VGB6 | C57BL/6NTac | |
| 10416A-E2 | VGB6 | C57BL/6NTac | |
| 10416B-H7 | VGB6 | C57BL/6NTac | |
