| Gene | Apof |
| Chromosome | 10 |
| Deletion Start | 128,268,737 |
| Deletion End | 128,270,032 |
| Deletion Size | 1,296 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 128,269,013 | 128,269,032 | TGCATAGCCTCCGACTCATC |
| TUP | 128,269,040 | 128,269,066 | CAATCCAGCTGCTTTGCTATCTCCTGC |
| TUR | 128,269,088 | 128,269,107 | ACTGGTTGCCTCTCCGTGTG |
| TDF | 128,269,410 | 128,269,428 | TGCCCTGTCCTCTGCTCTG |
| TDP | 128,269,430 | 128,269,452 | AGCTCTTAGCCTGGGAGCAGCCA |
| TDR | 128,269,476 | 128,269,497 | GTCGCAGTCTGTGTTGGAAATC |
PCR Primers
| Primer | Sequence |
| SU | TCACTGTTGAACCTTTCTGTC |
| SD | TGAAGGCACCTAGGACTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 335 bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | 316 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10417A-D6 | VGB6 | C57BL/6NTac | |
| 10417A-H2 | VGB6 | C57BL/6NTac | |
| 10417B-H8 | VGB6 | C57BL/6NTac | |
