| Gene | 1810049H13Rik |
| Chromosome | 11 |
| Deletion Start | 120,458,016 |
| Deletion End | 120,456,732 |
| Deletion Size | 1,285 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,457,523 | 120,457,544 | CTGGCTATCCGATCTGGACTTG |
| TUP | 120,457,494 | 120,457,521 | TCCTCCTGCGAACTTTGAGAGTGAATGC |
| TUR | 120,457,472 | 120,457,490 | TCGGCTCAATGCCTAACAC |
| TDF | 120,456,953 | 120,456,971 | GCTGCCACCTGAGCTTGAG |
| TDP | 120,456,927 | 120,456,950 | TCCCACCAACTGCTGCATGAGTGG |
| TDR | 120,456,884 | 120,456,904 | CAGCAGAGCTTCGGCATAATC |
PCR Primers
| Primer | Sequence |
| SU | TGTCCCGAGCCTCTAAGAC |
| SD | GGAAAGCTGTAGCTGTTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 307 bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | 328 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10420B-C5 | VGB6 | C57BL/6NTac | |
| 10420B-C8 | VGB6 | C57BL/6NTac | |
| 10420B-D3 | VGB6 | C57BL/6NTac | |
| 10420B-E12 | VGB6 | C57BL/6NTac | |
| 10420B-F3 | VGB6 | C57BL/6NTac | |
| 10420B-H9 | VGB6 | C57BL/6NTac | |
| 10420C-B8 | VGB6 | C57BL/6NTac | |
