| Gene | Unc119 |
| Chromosome | 11 |
| Deletion Start | 78,343,553 |
| Deletion End | 78,348,779 |
| Deletion Size | 5,227 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,344,215 | 78,344,232 | AGCCCTCACTCTGCTTCC |
| TUP | 78,344,236 | 78,344,260 | AGGAAGGCTGAGGCCATACTCTGCC |
| TUR | 78,344,264 | 78,344,284 | GCACCCGTATCAGCAATGTTC |
| TDF | 78,348,041 | 78,348,058 | TTGGCGTGGTGGCTACTC |
| TDP | 78,348,060 | 78,348,085 | CAGCCCTGCCCACTTCTCTTAACTCA |
| TDR | 78,348,091 | 78,348,112 | GCTTGTCTCCCACTGTGAACTC |
PCR Primers
| Primer | Sequence |
| SU | GAGGAGAGGTATTCGGTAAC |
| SD | TCTTACAGGCTGCGATCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 388 bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 411 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10425A-A8 | VGB6 | C57BL/6NTac | |
| 10425A-A12 | VGB6 | C57BL/6NTac | |
| 10425A-E9 | VGB6 | C57BL/6NTac | |
