| Gene | Flrt3 |
| Chromosome | 2 |
| Deletion Start | 140,661,703 |
| Deletion End | 140,659,702 |
| Deletion Size | 2,002 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 140,660,978 | 140,660,995 | ACAGCAGCGCCAGTGAAC |
| TUP | 140,660,954 | 140,660,976 | TTCCCGGCACAAGCCTGAGGAAG |
| TUR | 140,660,922 | 140,660,944 | ACCCGGTTGATATGGTTGTCTTG |
| TDF | 140,660,035 | 140,660,052 | TGCATAGGAACGGGTCAC |
| TDP | | | |
| TDR | 140,659,970 | 140,659,990 | ACCGGCTTCTGCATAGTCATC |
PCR Primers
| Primer | Sequence |
| SU | GTATATTCCAAAGACACTCAGG |
| SD | TGTGTTCAGGCATCCCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 375 bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 265 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10430A-C6 | VGB6 | C57BL/6NTac | |
| 10430A-D12 | VGB6 | C57BL/6NTac | |
| 10430A-E12 | VGB6 | C57BL/6NTac | |
| 10430A-G6 | VGB6 | C57BL/6NTac | |
