| Gene | 4930547C10Rik |
| Chromosome | 4 |
| Deletion Start | 52,610,099 |
| Deletion End | 52,612,620 |
| Deletion Size | 2,522 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,610,612 | 52,610,631 | TAGCCTGCACCGACTGATTC |
| TUP | 52,610,632 | 52,610,656 | CCTGGCTGAAACGAGAACTCACAGC |
| TUR | 52,610,662 | 52,610,685 | TCACTGTGTAACCATAGTCTCCAC |
| TDF | 52,611,824 | 52,611,842 | CCCAGTCCACCATGACCAG |
| TDP | 52,611,853 | 52,611,882 | CAGCTGGAAAGAATTGCAAATGTGCATCCA |
| TDR | 52,611,888 | 52,611,911 | TCCTCCAGTTTGAGATTCACCTTC |
PCR Primers
| Primer | Sequence |
| SU | ACTGCCTCGTATTCACTGTG |
| SD | GGTAAGACAGTAGTCCTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 341 bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | 375 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10435A-C8 | VGB6 | C57BL/6NTac | |
| 10435A-E8 | VGB6 | C57BL/6NTac | |
| 10435A-F9 | VGB6 | C57BL/6NTac | |
| 10435A-G5 | VGB6 | C57BL/6NTac | |
| 10435A-G9 | VGB6 | C57BL/6NTac | |
| 10435A-G3 | VGB6 | C57BL/6NTac | |
