| Gene | Gkn1 |
| Chromosome | 6 |
| Deletion Start | 87,350,869 |
| Deletion End | 87,346,230 |
| Deletion Size | 4,640 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,350,180 | 87,350,201 | GGCTGCGTTTCTCTTCTAGTTC |
| TUP | 87,350,152 | 87,350,179 | TGTCTAGAGGAGTGATTCTCGACCTTCC |
| TUR | 87,350,101 | 87,350,119 | GGCTGGGAGTCACGACAAC |
| TDF | 87,347,149 | 87,347,167 | TCCGTCAACCCTACCAGAG |
| TDP | 87,347,123 | 87,347,148 | TGGAGGACCTGAATACATTCGGACCA |
| TDR | 87,347,070 | 87,347,090 | GGAATCTCCTCGGCCACATAG |
PCR Primers
| Primer | Sequence |
| SU | GTCAGAGGATGAGTCAGAGG |
| SD | CAGCAGTGGCCTATCTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 342 bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | 326 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10439A-C4 | VGB6 | C57BL/6NTac | |
| 10439A-H8 | VGB6 | C57BL/6NTac | |
| 10439B-D8 | VGB6 | C57BL/6NTac | |
| 10439B-F11 | VGB6 | C57BL/6NTac | |
