| Gene | Saa4 |
| Chromosome | 7 |
| Deletion Start | 46,731,693 |
| Deletion End | 46,729,487 |
| Deletion Size | 2,207 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,731,482 | 46,731,499 | GGCAGGAGTCTGGCTTTC |
| TUP | 46,731,457 | 46,731,479 | TGGCTTCCACCCTACACTGGTCA |
| TUR | 46,731,406 | 46,731,425 | GCAGGCTGGACATTGTGAAC |
| TDF | 46,730,459 | 46,730,480 | ACAGCCCAGGACACTGTAACTG |
| TDP | 46,730,432 | 46,730,454 | TCAACCCAACCCAAGCCAACCCA |
| TDR | 46,730,398 | 46,730,419 | GTTCCCTGGTGACCATAAGGAG |
PCR Primers
| Primer | Sequence |
| SU | ATGATCAACTGACTCGTGGC |
| SD | AACAGAGACCGGTTGTGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10441A-B6 | VGB6 | C57BL/6NTac | |
| 10441A-E8 | VGB6 | C57BL/6NTac | |
| 10441A-G7 | VGB6 | C57BL/6NTac | |
| 10441A-G9 | VGB6 | C57BL/6NTac | |
| 10441A-G12 | VGB6 | C57BL/6NTac | |
