| Gene | Tmem215 |
| Chromosome | 4 |
| Deletion Start | 40,473,928 |
| Deletion End | 40,474,633 |
| Deletion Size | 706 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 40,474,191 | 40,474,211 | GGAAGTGGTGGAACTCCTGAG |
| TUP | 40,474,215 | 40,474,238 | CCCATCAGACCTGGAGTCAGGCAA |
| TUR | 40,474,249 | 40,474,271 | CCTGCCTTCTTAGCCAGTTCATC |
| TDF | 40,474,462 | 40,474,481 | AAGTGCTCAGCCTGGGACAG |
| TDP | 40,474,482 | 40,474,503 | ATCTGACCGCCCTGAGCCCGAG |
| TDR | 40,474,536 | 40,474,556 | TGCTTGTAGGAGCAAACGATG |
PCR Primers
| Primer | Sequence |
| SU | GTCAGAGATAGCAAGAAAGAG |
| SD | CTGTCTCTTCCCAGTGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 279 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | 314 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10442A-F6 | VGB6 | C57BL/6NTac | |
| 10442A-F5 | VGB6 | C57BL/6NTac | |
| 10442A-G5 | VGB6 | C57BL/6NTac | |
| 10442A-F7 | VGB6 | C57BL/6NTac | |
| 10442A-G8 | VGB6 | C57BL/6NTac | |
