Gene | Cyb5d1 |
Chromosome | 11 |
Deletion Start | 69,395,290 |
Deletion End | 69,393,629 |
Deletion Size | 1,662 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 69,394,548 | 69,394,570 | ACACGACAGAGGAAAGCAGATGG |
TUP | 69,394,521 | 69,394,547 | TCCTGGTACCCAGAGTTGGAAATTCCA |
TUR | 69,394,471 | 69,394,492 | GGCCTAGAGCTATGAAGGAGAC |
TDF | 69,394,301 | 69,394,322 | CCTTACCTGGGACTGTGGGATG |
TDP | 69,394,270 | 69,394,298 | TGAAGAGTGCTATCTGGTAATGGAGGCAC |
TDR | 69,394,244 | 69,394,268 | CCAGAAATGACAGAGCTTGAATCAG |
PCR Primers
Primer | Sequence |
SU | CGGACTGTCTTCATTTCCTC |
SD | GAGACTCTAGCTCTCTTACAG |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | 400 bp | - | + | + |
TUF | TUR | 100 bp | + | + | - |
TDF | TDR | 79 bp | + | + | - |
NeoFwd | SD | 335 bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
10451A-D6 | VGB6 | C57BL/6NTac | |
10451B-G2 | VGB6 | C57BL/6NTac | |
10451B-H12 | VGB6 | C57BL/6NTac | |
10451C-D8 | VGB6 | C57BL/6NTac | |