| Gene | Cyb5d1 |
| Chromosome | 11 |
| Deletion Start | 69,395,290 |
| Deletion End | 69,393,629 |
| Deletion Size | 1,662 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,394,548 | 69,394,570 | ACACGACAGAGGAAAGCAGATGG |
| TUP | 69,394,521 | 69,394,547 | TCCTGGTACCCAGAGTTGGAAATTCCA |
| TUR | 69,394,471 | 69,394,492 | GGCCTAGAGCTATGAAGGAGAC |
| TDF | 69,394,301 | 69,394,322 | CCTTACCTGGGACTGTGGGATG |
| TDP | 69,394,270 | 69,394,298 | TGAAGAGTGCTATCTGGTAATGGAGGCAC |
| TDR | 69,394,244 | 69,394,268 | CCAGAAATGACAGAGCTTGAATCAG |
PCR Primers
| Primer | Sequence |
| SU | CGGACTGTCTTCATTTCCTC |
| SD | GAGACTCTAGCTCTCTTACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 400 bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 335 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10451A-D6 | VGB6 | C57BL/6NTac | |
| 10451B-G2 | VGB6 | C57BL/6NTac | |
| 10451B-H12 | VGB6 | C57BL/6NTac | |
| 10451C-D8 | VGB6 | C57BL/6NTac | |
