| Gene | Smcr7 |
| Chromosome | 11 |
| Deletion Start | 60,730,273 |
| Deletion End | 60,732,034 |
| Deletion Size | 1,762 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,730,919 | 60,730,937 | AGCCCACGCCCATTCATTC |
| TUP | 60,730,938 | 60,730,961 | TCCCACGACACCTAAGTTCAGCAC |
| TUR | 60,730,971 | 60,730,988 | TGGAACGTCAGGCACAGG |
| TDF | 60,731,851 | 60,731,868 | AGCCTGCCCTGTCACTTC |
| TDP | 60,731,872 | 60,731,899 | CCCAGTGTGAACCTCTTGGGTAACTTCC |
| TDR | 60,731,905 | 60,731,926 | AGCCAATGTCATCAATCTCCTC |
PCR Primers
| Primer | Sequence |
| SU | GAAAGGCCTGGATCCTTCC |
| SD | GAGTTCATTGCTGGTGATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 315 bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 335 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10452B-F6 | VGB6 | C57BL/6NTac | |
| 10452C-A9 | VGB6 | C57BL/6NTac | |
