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Sequence Downloads and Strategy Information
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 103,218,074 | 103,218,096 | TGGTGGGTTTGGGATTTAATGTC |
| TUP | 103,218,048 | 103,218,072 | TTGCAAAGGTGGTCGCCGAATCCAT |
| TUR | 103,218,000 | 103,218,024 | TGGGTTTACTAGCTTTGGCTATCTG |
| TDF | 103,208,896 | 103,208,918 | AGCATGGCTCACAAATCTTGGAC |
| TDP | 103,208,871 | 103,208,895 | TCGGGAAACCAGGAATGTAGCTTGC |
| TDR | 103,208,820 | 103,208,841 | CCCTTGGATGGTAGCTGTCTTG |
PCR Primers
| Primer | Sequence |
| SU | ACCTTCAAGTTTCCTACATGC |
| SD | GTTAGGAGGCCTGAATCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 300 bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | 334 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the KOMP Repository.| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10454A-G8 | VGB6 | C57BL/6NTac | |
| 10454B-D9 | VGB6 | C57BL/6NTac |
