| Gene | Rangrf |
| Chromosome | 11 |
| Deletion Start | 68,973,715 |
| Deletion End | 68,972,601 |
| Deletion Size | 1,115 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 68,973,366 | 68,973,386 | GGCTGGACGGTTATTCAGGAC |
| TUP | 68,973,336 | 68,973,359 | TCCCAAGGGCAATAGCAGACCAGG |
| TUR | 68,973,277 | 68,973,298 | GCATGGGAGGAACAGTGGTAAG |
| TDF | 68,972,748 | 68,972,771 | TTCCTTCTCCCTCTATTCCCTTAC |
| TDP | 68,972,720 | 68,972,744 | AAGGTGTCACAGCAGGTCTCTTGGC |
| TDR | 68,972,690 | 68,972,710 | GCTCCAAGGTGGACATGACAG |
PCR Primers
| Primer | Sequence |
| SU | GCCGGGCTTCAGAGACTAG |
| SD | CTCCCTGCTCCTATACACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 341 bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 314 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10456A-B12 | VGB6 | C57BL/6NTac | |
| 10456A-C10 | VGB6 | C57BL/6NTac | |
| 10456A-C12 | VGB6 | C57BL/6NTac | |
| 10456A-G6 | VGB6 | C57BL/6NTac | |
| 10456A-G12 | VGB6 | C57BL/6NTac | |
