| Gene | Rilp |
| Chromosome | 11 |
| Deletion Start | 75,510,130 |
| Deletion End | 75,512,851 |
| Deletion Size | 2,722 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,511,045 | 75,511,066 | TCACAGCATTCAGCAGTTGATG |
| TUP | 75,511,068 | 75,511,090 | CCCAGCCTGGGTTTACCTGGCAT |
| TUR | 75,511,119 | 75,511,140 | GTTGCTACCTTCCAACTTCCTG |
| TDF | 75,512,537 | 75,512,555 | GTCTTCCCACCTGCTTCTC |
| TDP | 75,512,558 | 75,512,583 | TCCTCTGTGCCTTCATTTCCCTGTGA |
| TDR | 75,512,584 | 75,512,605 | GGAGGAAAGAGGACAGCATAGG |
PCR Primers
| Primer | Sequence |
| SU | GGATACCTGCCTGGAAGTTG |
| SD | GAAAAGCAGAGCTCAGAGATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 334 bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | 303 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10459A-F3 | VGB6 | C57BL/6NTac | |
| 10459A-F5 | VGB6 | C57BL/6NTac | |
| 10459B-C4 | VGB6 | C57BL/6NTac | |
| 10459B-G9 | VGB6 | C57BL/6NTac | |
| 10459B-D2 | VGB6 | C57BL/6NTac | |
| 10459B-H7 | VGB6 | C57BL/6NTac | |
