| Gene | Enam |
| Chromosome | 5 |
| Deletion Start | 88,488,719 |
| Deletion End | 88,504,753 |
| Deletion Size | 16,035 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,489,574 | 88,489,592 | ACGCCTGTGTTGGGTCTTC |
| TUP | 88,489,593 | 88,489,620 | CCTTTGCACTCTGTTGTTGCTTGACTCA |
| TUR | 88,489,645 | 88,489,667 | GAGGGAGAAATGCAGAGCCTTAG |
| TDF | 88,504,315 | 88,504,336 | GAGCAGCTCGTCATTGGTATTC |
| TDP | 88,504,338 | 88,504,363 | TGATAAAGGCTCTGGCCCAGATAGCA |
| TDR | 88,504,402 | 88,504,423 | TGATGGTAGGTGGCCTTTGTTG |
PCR Primers
| Primer | Sequence |
| SU | CTCTCTGATTTAGCTCTCATC |
| SD | TACTGGTGTGCAGATCTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 376 bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | 441 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10463A-A10 | VGB6 | C57BL/6NTac | |
| 10463A-H5 | VGB6 | C57BL/6NTac | |
| 10463A-A1 | VGB6 | C57BL/6NTac | |
| 10463B-A10 | VGB6 | C57BL/6NTac | |
| 10463B-D11 | VGB6 | C57BL/6NTac | |
| 10463A-E12 | VGB6 | C57BL/6NTac | |
