| Gene | Tmem115 |
| Chromosome | 9 |
| Deletion Start | 107,534,482 |
| Deletion End | 107,538,074 |
| Deletion Size | 3,593 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,535,210 | 107,535,231 | CGGCCTCCTGGTGAAAGTAAAG |
| TUP | 107,535,234 | 107,535,259 | ATGCCAGAAGACAGTGAAGCGCTACG |
| TUR | 107,535,300 | 107,535,319 | CCGCATCTTGAGGGTCTGTG |
| TDF | 107,537,496 | 107,537,517 | TAACCCTGCCCTTGGAAATGAC |
| TDP | 107,537,518 | 107,537,540 | ACCAGCGCACGTGGGACGCATTT |
| TDR | 107,537,574 | 107,537,593 | AAAGACGGGTGGGTGTAGTG |
PCR Primers
| Primer | Sequence |
| SU | ACCCAGGGCCTAACGACAG |
| SD | ATCTGCACTGGCTGCTGCT |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 349 bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | 317 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10466C-C4 | VGB6 | C57BL/6NTac | |
